Chromatography tags are used to alter chromatographic properties of the protein to afford different resolution across a particular separation technique.
[3] Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species.
These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Therefore, after purification, tags are sometimes removed by specific proteolysis (e.g. by TEV protease, Thrombin, Factor Xa or Enteropeptidase) or intein splicing.
(See Proteinogenic amino acid#Chemical properties for the A-Z amino-acid codes) HiBiT-tag was developed by Scientists at Promega.