The composition of the States was determined in Royal Decrees published on 9 and 11 November, and its members were first assembled on 15 December 1840.

Among the cities were Rotterdam (represented by ten members), The Hague (eight), Leiden (five), Dordrecht (four), Delft (three), Gorinchem, Gouda, Schiedam (two each), Brielle, Maassluis, Schoonhoven, Vlaardingen and Woerden (one each).

[1] In this period, the States of South Holland were responsible for electing the province's 12 members of the 58-member House of Representatives.

New members still took office on 1 July, but elections were now held on the second Tuesday in May, while by-elections had to be scheduled no later than two months after the seat in question became vacant.

[2] Prominent members of the States of South Holland have included Suze Groeneweg, Dirk Jan de Geer and Pieter Oud.