It forms a regular part of Jewish, Catholic, Lutheran, Anglican and other Protestant liturgies.

This psalm is quoted or referred to in several places in the New Testament: In the Eastern Orthodox Church, Psalm 68 (Psalm 69 in the Masoretic Text) is part of the ninth Kathisma division of the Psalter, read at the Midnight Office on Saturday mornings, at Vespers on Tuesday evenings, as well as on Mondays and Thursdays during Lent, at the Ninth Hour and the First Hour, respectively.

[22] In the Church of England's Book of Common Prayer, Psalm 69 is appointed to be read on the evening of the 13th day of the month,[23] as well as at Evensong on Good Friday.

[24] Heinrich Schütz set Psalm 69 in a metred version in German, "Gott hilf mir, denn das Wasser dringt", SWV 166, as part of the Becker Psalter, first published in 1628.

The King James Version of verse 20 of Psalm 69 is cited as text in the English-language oratorio "Messiah" by George Frideric Handel (HWV 56).