Puerto Pailas is a canton and town in the Santa Cruz Department in the South American Andean Republic of Bolivia.

It is situated at an elevation of 297 m on the left banks of Río Grande, one of the longest rivers in the Bolivian lowlands, which is 1,200 m wide at this place and is spanned by a road bridge here.

It sits a strategically important place within the region's road network, located 47 kilometers east of the departmental capital Santa Cruz, on the principal route from that city to Beni, the Chiquitanía, and Brazil.

[1] From Santa Cruz the tarmac road Ruta 4/Ruta 9 goes east through Cotoca to Puerto Pailas where it crosses the Río Grande and reaches Pailón on the river's eastern banks.

At Puerto Pailas, a dirt road leaves the Rutas 4 and 9 in north-westerly direction and goes 4 km to the neighbouring town Montero Hoyos.