Pukou District (simplified Chinese: 浦口区; traditional Chinese: 浦口區; pinyin: Pǔkǒu Qū), is one of 11 districts of Nanjing, the capital of Jiangsu province, China, lying northwest across the Yangtze River from downtown Nanjing.

The district was formerly the southern terminus of the Tianjin-Pukou Railway; railcars had to be ferried across the river until it was bridged in 1968.

It is separated from the main part of the city by the Yangtze River with hundred miles of mountains and forests.

The region also boasts beautiful natural landscapes and many places of interest including National Ancient Mountain and Forest Park, Pearl Spring, Tang Spring, Amber Spring, Damo Stela, Huji Temple Site, Doulv Temple, Wen Temple and old ginkgo trees.

Pukou District has been the transportation intersection between the northern and southern regions, as well as the Gate of the North in Nanjing city.