The Q65 bus route constitutes a public transit line in Queens, New York City.

The line proceeded south for five miles along an undeveloped right-of-way owned by the railroad, which would later become 164th Street, to what is now Normal Road, a few blocks north of Hillside Avenue.

[1][15] The current Q65 service begins at the College Point Line's terminal at 110th Street and 14th Avenue, and follows the former trolley route to Northern Boulevard.

The then-villages of Flushing and College Point granted franchises to the company in summer 1887, with the provision of only employing overhead trolley wire for five years before switching to battery power.

[1][2] Earlier that year on October 13, the Long Island Electric Railway (LIER), operators of the Jamaica−Far Rockaway Line, was purchased by the company.

[2] During the month of May in 1902, the Flushing–Jamaica Line was bought out by the parent New York and Queens company, through several complex proceedings and reorganizations.

The administration of Mayor Fiorello H. La Guardia, and New York City Parks Commissioner Robert Moses, also desired to use the right-of-way to build the planned Grand Central Parkway (this highway would instead be built along the western end of Flushing Meadows–Corona Park).

[24][25] On July 2, the railroad turned over the right-of-way of the Flushing–Jamaica Line between Flushing Cemetery and Jamaica to the city in order to create a proper 164th Street.

[1][10] Service on the College Point trolley was abandoned on August 23 of that year, replaced by buses between 110th Street and Flushing.

That year, the company applied for an extension of the route north along 122nd Street (College Point Boulevard), which was never implemented.

[30] In 2004, the southern termini of the Q65, Q25, and Q34 were moved west one block along Jamaica Avenue, from 160th Street to Parsons Boulevard.

[35] On April 15, 2013, Q65 Limited service began skipping two stops along College Point Boulevard, at 26th Avenue and the Whitestone Expressway, due to low ridership.

[40] In September 2015, as part of the Northeast Queens Bus Study, it was suggested to modify a small portion of the Q65 route near Flushing Cemetery, taking it off Bowne Street and moving it onto the wider Parsons Boulevard.

[40] In September 2016, because Q65 buses frequently detoured to avoid traffic on the narrow 14th Road, the Q65 was rerouted to run via 14th Avenue in College Point.

[47] As part of the new plan, the Q65 would still run to Flushing, but service to College Point would be replaced by an extension of the Q27 bus.

[49][50] The Q65 would run to Flushing, but service to College Point would be replaced by an extension of the Q26 bus, and the route would be modified in Murray Hill to avoid narrow streets.

[54] On January 29, 2025, the current plan was approved by the MTA Board, and the Queens Bus Redesign will go into effect by Labor Day.