It is underneath Uptown, Brisbane (formerly the Myer Centre) & Queen Street Mall.

Queen Street bus station serves as the terminus of many routes servicing the South East Busway, Southern and Eastern Suburbs and the Centenary / Indooroopilly / Kenmore corridor.

After stopping at King George Square, a number of inbound services bypass the Queen Street stop or terminate at the Cultural Centre on the southern side of the Brisbane River, and vice versa.

However, King George Square and Queen Street are only a short walking distance apart.

The station is divided into several platforms originally named after Australian native animals, but are now identified with letters and numbers: Passengers wait behind automatic doors on the stop that is assigned to their bus.