The R101 is a Regional Route in South Africa that is the designation for some of the old sections of roads that were previously the N1, prior to upgrading.

Its continues as the Old Johannesburg Road through the southern part of Centurion (where it crosses to the west of the Ben Schoeman Freeway, which is still designated as the N1).

It then flies over the N14 highway and passes through the western side of Centurion (where it meets the north-eastern terminus of the R114 route) before changing names again to Jan Smuts Drive in Pretoria, where it merges with the northern endpoint of the Ben Schoeman Freeway (which is now labelled as the N14) at Kgosi Mampuru Street (formerly Potgieter Street[4]), just after the Voortrekker Monument.

As Paul Kruger Street, the R101 goes north, passing the Pretoria Zoo, through Capital Park and Mayville.

After crossing the N4, the R101 becomes Old Warmbaths Road and follows the Apies River north-north-east for 27 km, through Pyramid, to Hammanskraal.

After Kransvlei Farm Resort, the R101 continues by way of a left turn at the t-junction to become the straight road through Mookgophong (Namboomspruit).

Passing through Mookgophong as Thabo Mbeki Street, it intersects with the R519 at Second Avenue and the R520 at Nelson Mandela Drive.

After the Mokopane Game Breeding Centre, the R101 turns eastwards to cross the bypassing N1 highway at its Mokopane North Off-ramp (Sebetiela Ramp Toll Plaza) and follow it north-east to Polokwane (40 km) while being on its eastern side.

[6][7] The point where Landdros Mare Street and the Polokwane Eastern Bypass meet north of Polokwane marks the end of the R101, as the road heading north to the towns of Louis Trichardt, Musina and the border of Beit Bridge with Zimbabwe is given back to the N1.