The R539 is a Regional Route in Mpumalanga, South Africa.

It connects the R36 between Waterval Boven and Mashishing (Lydenburg) with the R37 between Sabie and Nelspruit.

[1] Its western origin is the R36 between Waterval Boven and Mashishing (Lydenburg), at the Chom se Hoogte Pass.

It runs east through De Beersnek Pass, Patatanek, and Schoemanskloof pass[2] to join the N4.

The R539 is cosigned with the N4 through the Montrose pass, but it leaves the N4 on the eastern side, heading north-east to end at the R37 between Sabie and Nelspruit.