The R541 is a Regional Route in Mpumalanga, South Africa that connects Machadodorp with Lochiel.

[1] Its north-western origin is the R36 near Machadodorp, Mpumalanga.

From there it heads south-east as the Skurweberg Pass, to meet the R38.

It is co-signed for 6 kilometres heading south-west to Badplaas.

From Badplaas it diverges and again heads south-east, ending in Lochiel at an intersection with the N17.