Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA).
[1][2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.
The stronger detergents in RIPA buffer (such as SDS) cause greater protein denaturation and decrease protein-protein interactions.
RIPA buffer recipes vary slightly between authors and may include: The following ingredients are optional and included as needed:
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