She was credited as Gracy in the Malayalam movies she starred in her career since her debut film Bharya (1962), directed by Kunchako.
Rajasree went on to do 200 films spread across the languages – Telugu, Tamil, Kannada, Malayalam (as Gracy) and Hindi from beginning of 1956 to 1979.
She worked as child artiste in films like Bhaktha Ambarisha (1959) and Mangalyam (1960) in Telugu, Aasha Sundari (1960) in Kannada, Petraval Kanda Peruvazhvu (1960) and Baktha Sabari (1960) in Tamil.
However, she was cast only as supporting and second lead actress in Tamil films like Sengamala Theevu (1962), Nichaya Thaamboolam (1962), Thendral Veesum (1962), Kalai Arasi (1963), Yarukku Sondham (1963) and Kattu Maina (1963) in the period 1962 to 1963.
Her notable films in Malayalam as the lead heroine include Jail (1966), Kadamattathachan (1966), Anarkali (1966), Udhyogastha (1967), Karutha Rathrikal (1967), Kadal (1968), Dial 2244 (1968), Urangatha Sundary (1969), Sree Guruvayoorappan (1972), Sreemurukan and Devi Kanyaakumaari (1974).
She quoted in an interview "When I got a call from such a big banner as Chitralaya asking me to be present at the studio the next morning, I couldn't believe my ears.
Hence both during the shooting of Bama Vijayam and every time she met actor Nagesh, he would always call her as Dhiglazhagi as in the film, a thief refers to her by that name.
It was decided by Sridhar to remake Kadhallikaa Neram Illai in Hindi as Pyar Kiye Jaa and he retained only Rajasree from the original.
The pair Rajasree-Kanta Rao worked in Pakkalo Ballem (1965), Bangaru Timmaraju (1964), Saptaswaralu (1969), Pratigyna Palana (1966), Vijaya Simha (1965), Aggidora (1967), Bhoolokamlo Yamolokam (1966), Devudichchina Bharta (1968), Veera Pooja (1968), Ranabheri (1968), Aggimeeda Guggilam (1968), Oke Kutumbham (1970), Merupu Veerudu (1970), Maa Manchi Akkayya (1970), Prema Jeevulu, Bhale Ettu Chivaraku Chittu (1970) and Raite Raju (1970).
She played second lead supporting roles in Kannada films like Swarna Gowri, Rathna Manjari, Karuneye Kutumbada Kannu and Chandra Kumara.
Her Kannada films as lead heroine include Chandavalliya Thota, Satya Harishchandra (1965), Bala Nagamma (1966), Bhale Basava, Mr. Rajkumar (1970), Jwala Mohini, CID 72 and C.I.D.
Her other notable films in Tamil include Kallum Kaniyagum, suspense thriller Selva Magal (1967), Siritha Mugam (1968), Patham Pasali (1970), Poi Sollathae (1971), Pattondru Ketten (1971), Yanai Valartha Vanampadi Magan, Amudha (1975) and Veeduvarai Uravu (1976).