Sir Ralph Rowlett (by 1513 – 20 April 1571), of Holywell House, St Albans, Hertfordshire, was an English politician.
He was the eldest son of Ralph Rowlett of London and St Albans by his first wife Jane Knight.
He married firstly Dorothy Bowles, the daughter of John Bowles of Wallington, Hertfordshire, and secondly Margaret Cooke (d. 3 August 1558), daughter of Sir Anthony Cooke of Gidea Hall, Essex.
He left a life interest in Holywell to Chief Justice Sir Robert Catlyn.
This article about a 16th-century member of the parliament of England is a stub.