Kolo Muani began his professional career at Nantes, where he scored 23 goals in 87 first-team appearances and won the Coupe de France.

[17] At Boulogne, he demonstrated his talents and contributed three goals and five assists, helping the club reach third place in the standings in the tournament, which ended prematurely due to the COVID-19 pandemic.

[23][24] On 4 March 2022, Bundesliga club Eintracht Frankfurt signed Kolo Muani on a pre-contract agreement ahead of the expiration of his Nantes contract.

[38] On 15 September, Kolo Muani made his PSG debut as a substitute in a 3–2 defeat to Nice, assisting Kylian Mbappé's second goal of the match.

[42] By the end of the season, he had scored nine goals and provided six assists in 40 games for PSG, contributing to successes in Ligue 1, the Coupe de France, and the Trophée des Champions.

[50] On 7 February, he scored another brace in a 2–1 away victory against Como, and by finding the net in his first three Serie A games, he matched a feat last achieved for the club by Roberto Baggio in 1990.

[53] On 16 November 2022, he replaced Christopher Nkunku in France's FIFA World Cup squad after the latter was forced to withdraw due to injury.

[54][55] On 14 December, Kolo Muani scored his first goal for France, in the semi-final match against Morocco, slotting in a deflected shot from Kylian Mbappé.

[58] During the match, past the 120th minute and into the final stoppage time before penalties, Kolo Muani had an opportunity to score the go-ahead goal and potentially win the World Cup for France, but it was saved by Emiliano Martínez in what has been described as the "new 'Save of the Century'".

[66] In the round of 16 against Belgium on 1 July, Kolo Muani provoked an own goal after his shot was deflected into the Belgian net by Jan Vertonghen, securing a 1–0 victory that put France in the quarter-finals.

Like Henry, Kolo Muani began his career as a wide player known for his pace and trickery, but eventually transitioned into a center-forward position.

France national team coach, Didier Deschamps, acknowledged Kolo Muani's many qualities, describing him as a player who has a significant presence, runs in behind from deep, and has a good finishing ability.

Frankfurt's head coach, Oliver Glasner, praised Kolo Muani's speed, tackling, dribbling, and finishing skills, noting that he is an asset to the team.

Markus Krösche, Eintracht Frankfurt's board member for sport, expressed his excitement for Kolo Muani's potential, stating that his speed, robustness, finishing skills, and tactical versatility are qualities that the team needs.