Regalbuto (Latin: Ameselum; Sicilian: Regarbutu) is a comune in the province of Enna, Sicily, southern Italy.

Regalbuto may be the ancient town of Amaselos, which was named by the Greek and Sicilian historian Diodorus Siculus in his Bibliotheca historica (Historical Library) writings.

In 1860, a number of members of parliament met in Regalbuto with Garibaldi to discuss a truce during the Campaign of 1860.

The town was never subject to fiscal taxes, and a free magistracy elected by the king and the archbishops of Messina governed it.

The town was severely damaged during World War II, and on August 3, 1943 the Pionier Battalion of Hermann Göring Panzer Division, supported by tanks and self-propelled guns was forced to cede the town, after four days of bitter fighting by the 1st Infantry Brigade of 1st Canadian Infantry Division.