Riekoperla darlingtoni is found on Mount Donna Buang, a mountain peak in the Victorian Central Highlands which lies in one of the oldest and highest areas of dissected Palaeozoic rocks (formed approximately 500 million years ago) in the Central Highlands of Victoria.

It apparently occurs only in the area within 1 kilometre (0.62 mi) of the summit of Mount Donna Buang, particularly on the southern slopes and is restricted to the small temporary streams which flow through forest dominated by alpine ash.

The adult stonefly lives within rolled pieces of alpine ash bark suspended in low vegetation along natural drainage courses and in the vicinity of streams.

Of particular scientific interest is the species' ability to survive the annual drying of its habitat, both in the egg stage and by burrowing down to a damper level and its seasonal flexibility in growth and emergence times, which enhances survival during the extremes of climate experienced on mountain tops.

The Mount Donna Buang Stonefly has recently been reassessed as Critically Endangered under the Victorian State legislation the Flora and Fauna Guarantee Act 1988.