On May 30, 1896, electric trolleys began service between East Main Street station in Rochester and Glen Haven.

Sodus Bay cars used Rochester Railway Company tracks to reach downtown and turnback at Plymouth Avenue.

The Irondequoit Park Railroad was leased by the Rochester and Sodus Bay Railway in 1901, and formally merged in 1902.

[3] Automatic block signals were installed along the Rochester and Sodus Bay line between 1913 and 1914, a welcome safety feature given the high density schedule that was operated at the time.

In 1925, Sodus Bay cars abandoned the Rochester downtown interurban terminal and instead originated from the Blue Bus Lines depot on South Avenue.

Cars 170 and 171 were temporarily assigned to Rochester Subway service until replacements were secured from the Utica Lines.

The line from Glen Haven east to Sodus Point was shut down on June 27, 1929, ending interurban service.

Due to better roads and increase automobile use, traffic on the Glen Haven line dropped off dramatically, and streetcar service ended on July 19, 1933.

Farmers along the 6 miles (9.7 km) of right-of-way not along a public highway purchased the alignment according to New York state law.

The old railway grade through the city limits was acquired by Rochester Gas & Electric for use as a transmission line right of way, and is easily followed today.

The body of Car 113 resides at the Seashore Trolley Museum in Kennebunkport, Maine, awaiting evaluation for future restoration.