The Diocese of Tlaxcala (Latin: Dioecesis Tlaxcalensis) is a Latin Church ecclesiastical territory or diocese of the Catholic Church in Mexico.

It is a suffragan in the ecclesiastical province of the metropolitan Archdiocese of Puebla de los Ángeles,[1] which was itself officially named the "Diocese of Tlaxcala" until 1903 (founded in 1519 and so named in 1525, the oldest diocese in Mexico).

[2] The present Diocese of Tlaxcala was erected on 23 May 1959 from territory of that archdiocese and the adjacent Archdiocese of Mexico.

[1] Its cathedra is found within the Cathedral of Our Lady of the Assumption in the episcopal see of Tlaxcala.

This article on a Roman Catholic diocese in Mexico is a stub.