Knott toured with Richard Mansfield's company in A Parisian Romance and Beau Brummel.

She succeeded Julia Marlowe in the role of Mary Tudor in When Knighthood was in Flower by Charles Major, from 1902 to 1903.

[6] In 1906, she played the title role in The Duchess of Devonshire, written for her by Elizabeth Johnson Ward Doremus.

[1][7] Knott was on stage in Logansport, Indiana in 1906, appearing in Alice Sit-by-the-Fire by James Barrie, when a fire began in the backstage.

In 1912 she toured North America in The Awakening of Helena Richie,[10] and reportedly set off "a sort of Roselle Knott epidemic", as the name Roselle was given to new babies in the cities she visited: "I like the name myself, and do not wonder that mothers give it to their girl babies," she told 1912 newspaper.