Rowland Louis Johnston (April 23, 1872 – September 22, 1939) was a U.S. Representative from Missouri's 16th congressional district.

Born in Louisiana, Missouri on April 23, 1872, Johnston attended the public schools.

He moved to Rolla, Missouri, in 1926 and continued the practice of law.

He resumed the practice of law in Rolla, Missouri, until his death there on September 22, 1939.

He remains were cremated and the ashes deposited in the mausoleum at Oak Grove Cemetery, St. Louis, Missouri.