Translational regulation of the stationary phase sigma factor RpoS is mediated by the formation of a double-stranded RNA stem-loop structure in the upstream region of the rpoS messenger RNA, occluding the translation initiation site.

Thus, at least two small RNAs, DsrA and RprA, participate in the positive regulation of RpoS translation.

[4] Wasserman et al. demonstrated that this RNA is bound by the Hfq protein.

[6] In the presence of Hfq the stability of RprA is influenced by the osmolarity of the cell, this is dependent on the endoribonuclease RNase E.[7] It has been shown the RprA regulates the protein coding genes, called csgD, this protein encodes a stationary phase-induced biofilm regulator and ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription.

These two target genes are repressed by RprA which results in regulation of biofilm formation.