She was popularly known as Nightingale of Sindh as she performed most of her songs in Sindhi, Urdu, Punjabi and Saraiki languages.

Rubeena was studying in class IX, when she gave audition at Radio Pakistan Hyderabad as a child singer.

Her first Sindhi song recorded for Radio was "Paren pawadee san, chawandi san, rahi waj rat bhanbhor men" (Sindhi: پيرين پوندي سان چوندي سان، رهي وڃ رات ڀنڀور ۾).

[9] Along with her singing career, she studied at the University of Sindh and received the Master of Arts degree in Muslim History.

[10] She also sang many marriage songs called "Sahera" along with her fellow singers Zarina Baloch, Amina and Zeb-un-Nissa.

After her marriage to famous Film and TV actor Mustafa Qureshi in 1970, she shifted to Lahore with her husband.