[5] As a young child, she travelled frequently, living in rural Victoria, Tasmania, and Surfers Paradise before finally settling back in Melbourne.

The collection, named Milk and Honey Designed by Ruby Rose,[8] includes washed jeans, leather jackets and T-shirts.

[5] As part of the competition, she downed 100 shots of beer in 100 minutes against Jackass's Bam Margera, and kissed strangers on a busy Sydney street.

[14] In July 2009, Rose along with Dave Hughes, Charlie Pickering, Carrie Bickmore and James Mathison hosted The 7pm Project, an Australian television news talk show produced by Roving Enterprises which airs weeknights on Network Ten.

In October 2015, Rose hosted the 2015 MTV Europe Music Awards alongside Ed Sheeran in Milan.

Rose credits her 2014 short film Break Free, which she produced herself, for the success of her acting career.

In an interview with Variety, she described how she was not able to get a manager, agent, or audition; so she decided to create short films "as a way of being able to give myself something to do and to study my craft."

[24] She was also cast in a guest role, as the service robot Wendy, in the science fiction series Dark Matter.

[citation needed] In 2016 and 2017, Rose appeared in three action film sequels, XXX: Return of Xander Cage, alongside Vin Diesel, Resident Evil: The Final Chapter as Abigail, and John Wick: Chapter 2, alongside Keanu Reeves; she also played a musical rival in the comedy Pitch Perfect 3, which was released in December 2017.

Some online commentators criticised Rose for not being Jewish, while the main focus of the scrutiny was the assertion that the fact she identifies as genderfluid made her "not gay enough" for the role.

[31] Prior to leaving Twitter, she released a tweet responding to the negative reaction, stating, "Where on earth did 'Ruby is not a lesbian therefore she can't be batwoman' come from — has to be the funniest most ridiculous thing I've ever read.

[31][33] In Entertainment Weekly's 2019 LGBTQ special issue, Rose additionally discussed the backlash to her casting as the title character of the television series Batwoman.

[35] Her character was subsequently written out of the series and replaced with a new one named Ryan Wilder, portrayed by Javicia Leslie.

[37] In August 2020, she called being the lead of a series "taxing" and stated her back surgery following the injury was a contributing factor in deciding to leave.

[42] A few months later, in October, she began to publish allegations of an abusive and dangerous working environment on the Batwoman set, naming Warner Bros. executive Peter Roth, costars Dougray Scott and Camrus Johnson, and others on the production as responsible parties.

[43][44][45] However, in response, Johnson confirmed that Rose had in fact been fired from Batwoman (not quitting as she previously claimed),[46] with Warner Bros. then releasing a statement responding to the accusations, saying that "Warner Bros Television had decided not to exercise its option to engage Ruby for season two" after "multiple complaints about her workplace behaviour".

[47] On 22 May 2020, Rose portrayed Viola in a livestreamed table-read of Twelfth Night as part of a benefit to raise money for COVID-19 pandemic relief.

Issues of concern to Rose include animal welfare, campaigns for anti-bullying and youth mental health, where she works as an ambassador for Headspace.

"[63][64] This announcement came approximately a week after she released Break Free, in which she visually transforms from a feminine-presenting woman to a heavily tattooed masculine-presenting person.

[77] The public and media attention increased following Rose's debut on Orange Is the New Black, significantly with regard to heterosexual women commenting on her physical appearance.

[80] Emma Teitel of Maclean's stated, "Rose ... resembles an androgynous Angelina Jolie; she is a rare combination of angular and soft.

"[78] Alex Rees of Cosmopolitan said that everyone was enamored of Rose at the time of her season three appearance on Orange Is the New Black.

[81][78] Rose acknowledged the public discourse concerning her physical appearance and subsequent debate on sexual fluidity after heterosexual women said they would "go gay" for her,[78] commenting that while she thought it was brilliant and was neutral on the matter, some found the public affection toward her inappropriate because they believe it implied people can choose to be gay.