The Rusishvili were a princely family, tavadi, in the eastern Georgian kingdom of Kakheti.
A genealogical tradition enshrined in the writings of Prince Ioann of Georgia (1768–1830) traces the family's ancestry to the late 12th century, making them descended from a Rus guardsman of Prince Yuri of Novgorod, the first husband of Queen Regnant Tamar of Georgia.
His progeny allegedly adopted the surname of Rusishvili, literally "children or scions of the Rus".
The family is included in the list of the Georgian nobility attached to the Russo-Georgian treaty of Georgievsk of 1783.
Members of the family occupied the office of Chief Treasurer at the court of the kings of Kakheti and, since 1724, the episcopal sea of Nekresi.