Rusticyanin

[5] Rusticyanin is also found in the membrane-bound form in the surface of T. ferrooxidans.

It is a part of an electron transfer chain for Fe(II) oxidation.

[4][6] As A. ferrooxidans can grow aerobically at pH values of 1.6 to 3.5, it obtains its energy for chemolithotrophic growth on soluble ferrous ions.

The mechanism of electron transfer in the respiratory oxidation pathway of Fe2+ in A. ferrooxidans is still unclear despite decades of research in this area.

[7] Predicted functional partners include coxD (cytochrome c oxidase, aa3-type, subunit IV, 64 amino acids), coxC (cytochrome c oxidase, aa3-type, subunit III, 184 amino acids), ctaB (protoheme IX farnesyltransferase), Cyc2 (cytochrome c, 485 amino acids), AFE_3142 (major facilitator family transporter, 397 amino acids), coxA (cytochrome c oxidase, aa3-type, subunit I, 627 amino acids), Cyc1 (cytochrome c552, 230 amino acids), and AFE_3141 (rrf2 family protein, 146 amino acids).