It is operated by Transdev Rheinland GmbH as a subcontractor of Regiobahn Fahrbetriebsgesellschaft mbH (Regiobahn for short), who is the PSO operator on behalf of Verkehrsverbund Rhein-Ruhr (VRR).

The line is operated using Integral S5D95 DMUs at 20-minute intervals,[2] with only two trains an hour running between Mettmann and Wuppertal.

[3] Until 2021, Bombardier Talent DMUs were used, which were replaced by second-hand Integral trains from the Bavarian Oberland Railway.

The planned electric Stadler FLIRT trains have already been built, and are currently used by Abellio Rail NRW on other lines of the Rhine-Ruhr S-Bahn.

[9] On 13 December 2020, the line was extended from Mettmann Stadtwald via Wuppertal-Vohwinkel to Wuppertal Hbf.