The line is operated at 20-minute intervals between Starnberg and Munich East.

In the peak hour services are extended to and from Zorneding every 20 minutes.

It is operated using class 423 four-car electrical multiple units, usually as two coupled sets.

In the evenings and on Sundays they generally run as single sets.

The section from Munich East to Zorneding (which is now normally operated by line S 4 services) was formerly operated as line S 5 between Herrsching and Ebersberg.