SABIO-RK comprises a reaction-oriented representation of quantitative information on reaction dynamics based on a given selected publication.

This includes reactions, their participants (substrates, products), modifiers (inhibitors, activators, cofactors), catalyst details (e.g. EC enzyme classification, protein complex composition, wild type / mutant information), kinetic parameters together with corresponding rate equation, biological sources (organism, tissue, cellular location), environmental conditions (pH, temperature, buffer) and reference details.

Data are adapted, normalized and annotated to controlled vocabularies, ontologies and external data sources including KEGG, UniProt, ChEBI, PubChem, NCBI, Reactome, BRENDA, MetaCyc, BioModels, and PubMed.

Several tools, databases and workflows in Systems Biology make use of SABIO-RK biochemical reaction data by integration into their framework including

MeMo-RK,[7] CellDesigner,[8] PeroxisomeDB,[9] Taverna workflows or tools like KineticsWizard software for data capture and analysis.