Sakai Masahisa (坂井 政尚, died November 26, 1573) was a Japanese samurai of the Sengoku Period, who most notably served the Oda clan.

Sakai was born in Mino Province, and first served the Saitō clan.

In late 1568, Masahisa joined Shibata Katsuie, Hosokawa Fujitaka, Hachiya Yoritaka, and Mori Yoshinari in attacking Iwanari Tomomichi's Shōryūji Castle.

Later in 1570, at Battle of Anegawa while under attack from Asai forces under Isono Kazumasa, he lost his son Sakai Kyūzō.

Family headship was thus inherited by his second son, Sakai Etchū no kami.