The club was founded in 1993, entering professional football in 1994.

It has spent most of its history playing between licensed.

In 2014 the club won its first title in the second division, but was unable to remain in the elite for a long time, withdrew from championship contention just a year later.

Samambaia won the second level once time in 2020, and in 2022, after being relegated again.

[1][2] Following is the summary of Samambaia appearances in Campeonato Brasiliense.