He played college football for the Florida State Seminoles and was selected by the Tennessee Oilers in the second round of the 1998 NFL draft.

However, in 2006 he struggled against the deep ball in several early games, most notably in week 6 against the Panthers where Rolle gave up two long touchdowns to Drew Carter and Steve Smith.

On November 21, 2007, Rolle disclosed that the illness was epilepsy due to years of giving and receiving crushing hits during his football career.

[3] He finished the season playing in only six games and ended the campaign with 22 tackles and one interception and won the "Ed Block Courage Award"..

Even though he made ten regular season starts with three interceptions in 2008, Rolle's injuries continued to limit his playing time.

He missed six games with shoulder and neck problems, and was inactive for the AFC championship playoff contest against the Pittsburgh Steelers due to a groin injury.

On June 13, 2007, Rolle was in South Africa on board a small commuter airplane returning from a safari when the door at the back of the plane flew off.

[7] Rolle was on the plane with his wife along with then-Philadelphia Eagles cornerback Lito Sheppard, then-Jacksonville Jaguars running back Fred Taylor, their respective wives and three other people.

Along with his wife, Danisha, and the Samari Rolle Foundation, in 2002, he kicked off a bowling tournament featuring his Titans teammates, members of the media and corporate sponsors.