Samuel Holten (June 9, 1738 – January 2, 1816) was an American Founding Father, physician, jurist, and politician from Danvers, Massachusetts.

Holten represented the Massachusetts Bay Colony as a delegate to the Continental Congress, where he signed the Articles of Confederation.

After numerous roles at the state and national levels in the 1780s, Holten was elected to a term in U.S. House of Representatives, serving from 1793 to 1794.

Holten was born in Salem Village (now Danvers) in the Province of Massachusetts Bay on June 9, 1738.

During the American Revolution, Holten actively supported the Patriot cause, serving in the militia as a major in the First Essex County Regiment.