Santi Bartolomeo e Stefano is a Baroque-style, Roman Catholic church located at number 1 Largo Belotti in Bergamo in the Lombardy region of Italy.

The church was built in 1613-1642, adjacent to a monastery of San Bartolomeo, which had belonged to the religious order of the Humiliati.

The chapel of the Madonna of the Rosary is highly decorated in stucco and painting (1752) by the studio of Antonio and Muzio Camuzio.

The cornices are decorated with 15 Holy Mysteries of the Rosary (1757) in monochrome by Giuseppe Antonio Felice Orelli.

He also frescoed (1749) in the choir and presbytery, the Sacrifice of Isaac and Glory of Santissimi Sacramento, but died before the completion of all of his designs.