San Giacomo degli Spagnoli is a basilica church in Piazza Municipio in central Naples, Italy.

The original church was commissioned in 1540 by the Spanish viceroy Don Pedro Álvarez de Toledo, Marquis of Villafranca and associated with the adjacent hospital for the poor.

The construction of the Palazzo San Giacomo did away with the facade, but retained the internal layout of three naves and a tall central ceiling.

The interior still retains a number of monumental tombs, including for the viceroy Don Pedro de Toledo, his wife and son, sculpted in 1570 by Giovanni da Nola.

In addition the tomb of Ferdinando Maiorca and his wife Porzia Coniglia in the apse was completed by Michelangelo Naccherino.