The timetable number of 335 applies to the whole route from Erfurt to Magdeburg, including the Sangerhausen–Erfurt section.

The line crosses the Unstrut and follows the river through the Diamantene Aue lowlands to the Thüringer Pforte (Thuringian Gate) at Heldrungen.

Beyond this gorge of the Unstrut, between the ridges of Hainleite to the west and Schmücke to the east, the line enters the Thuringian Basin.

The Regional-Express 10 (Magdeburg–Erfurt) service is operated by DB Regio with Siemens Desiro Classic (class 642) diesel multiple units and the Regionalbahn 59 (Sangerhausen–Erfurt) service is operated by Abellio Rail Mitteldeutschland with Talent 2 electric multiple units.

Since December 2015, Reinsdorf station has been served only during the evening and on weekends and public holidays.