[3]: 807 Santa Ana is the capital of the Yacuma Province and the Santa Ana del Yacuma Municipality, located at an elevation of 144 m above sea level, where the Yacuma River meets the Mamoré River.

Santa Ana is located 150 Kilometer north-west of Trinidad, the department's capital.

[4] The yearly precipitation of the region is 1,700 mm, with a distinct dry season from May to September.

Monthly average temperatures vary from 24 °C und 29 °C over the year.

According to the Köppen classification system Santa Ana del Yacuma has a Tropical savanna climate, abbreviated "Aw", bordering on a Tropical monsoon climate.