Santa Maria di Castello is a church and religious complex in Genoa, Italy.

Administrated for a long time by the Dominicans, it is located in the Castello hill of the city, where in the Middle Ages a bishop's fortified castle existed.

The high altar is decorated by a marble group of the "Assumption" by Domenico Parodi (late 17th century), while the chapel to the left of the presbytery has a Santa Rosa da Lima by Domenico Piola and a marble cover by Taddeo Carlone.

The loggia facing the second cloister has frescoes of Saints, a Madonna and, on the first floor, an Annunciation by Giusto d'Alemagna (1451).

In the upper floor has a statue of "St. Catherina of Alexandria" and a marble tabernacle attributed to Domenico Gagini (15th century).