Santi Bartolomeo e Gaetano is a Renaissance style, Roman Catholic church in central Bologna; it is located near the Two Towers, adjacent to the Strada Maggiore.

The church was designed by Giovanni Battista Falcetti with elaboration by Agostino Barelli, and owes the awkward facade due to its construction in 1517 at the site of a palace begun by Andrea da Formigine, commissioned by a member of the Gozzadini.

In 1599, the church came under the leadership of the Theatines, and in 1627, they ordained a complete restructuring of the complex by Giovanni Battista Natali, called il Falzetta, and by Agostino Barelli.

The 4th altar on right has an Annunciation (of the Beautiful Angel) (1632) by Francesco Albani and he also painted the lateral wall frescoes (1633) depicting a Nativity and Dream of Joseph.

The chapel of the left transept has a Beato Paolo Buralli (1772) by Ubaldo Gandolfi and a small Madonna with sleeping Jesus (1632) by Guido Reni.