Diesel and electric multiple units carried normal versions of the Regional Railways livery.

Initially, vehicles received the new ScotRail logo applied with vinyl stickers; a stylised outline of Scotland composed of three flashes in colours of green, red and purple.

The latter were replaced by ex-Stansted Skytrain Class 322 units which were never repainted under the National Express franchise, receiving only ScotRail logo transfers.

When the Class 322 hire ended in 2004, they were replaced on the North Berwick branch by English Welsh & Scottish Class 90 electric locomotives hauling former Virgin Trains West Coast Mark 3 coaching stock, in the old Virgin red and grey livery, again with ScotRail logo transfers.

[6] When FirstGroup became the franchisee on 17 October 2004, in line with other rail franchises it held, the brand was revised as First ScotRail.

[9][10] Transport Scotland designed with a new livery of dark blue background, with grey doors and a white dotted 'Saltire' Scottish flag.

A new logo was also applied, ScotRail, with the tagline 'Scotland's Railway', which on some units is replaced with the Scottish Gaelic translation 'Rèile na h-Alba'.