Semproniano is a comune (municipality) in the Province of Grosseto in the Italian region of Tuscany, about 120 kilometres (75 mi) south of Florence and about 35 kilometres (22 mi) east of Grosseto.

Semproniano borders the following municipalities: Castell'Azzara, Manciano, Roccalbegna, Santa Fiora, Sorano.

The municipality consists of the municipal seat of Semproniano and the villages of (frazioni) of Catabbio, Cellena, Petricci and Rocchette di Fazio.

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