The first station, Minami-Tatsumi, had them installed on March 14, 2014 and operation started in April.
The final station, Nodahanshin, had them installed and operating in December.
A southeastern extension to Mito Station in Higashiōsaka has been proposed since 1989.
ATO was previously trialled on the Sennichimae Line between October 1973 and July 1974.
Since 2016, the regular inspection of the trains used on the third rail lines of Osaka Metro has been consolidated at Midorigi Depot on the Yotsubashi Line, so a new spur track has been constructed near Hommachi Station for Sennichimae Line trains to access Midorigi Depot.