In a 2D experiment, cross peaks must be correlated to the correct nuclei.
Using sequential walking, the correct nuclei can be assigned to their crosspeaks.
The assigned crosspeaks can give valuable information such as spatial interactions between nuclei.
Each nucleotide has protons on the deoxyribose sugar, which can be assigned using sequential walking.
Knowing the DNA sequence helps, but in general the first nucleotide can be determined using the following rules.