It was opened in 1991[2] with the inauguration of the Madrid–Seville high-speed rail line, and serves around 9.25 million passengers a year.

As part of the preparations for the arrival of the Seville Expo '92, the rail tracks on the river bank were removed and the area redeveloped.

Santa Justa is the hub of the Cercanías Sevilla commuter rail network, and is served by AVE high-speed trains to Madrid and Barcelona via Córdoba, and southward AVE services to Málaga María Zambrano and Granada via Antequera-Santa Ana.

Alvia trains operate from Madrid to Cádiz via Santa Justa, as well as Larga and Media Distancia services to Algeciras, Almería, Huelva and Jaén.

The MetroCentro tram line is projected to be extended to serve Santa Justa station.