Because the bridge over the Goulburn River was not finished, the line from Essendon station had opened on 18 April 1872 to a temporary terminus at School House Lane.

School House Lane / Dysart Sidings / Goulburn Junction was located between Seymour and Tallarook stations.

[8] The interior of the station was altered to the current layout in 1997, when a general refurbishment was carried out, with the parcels office being converted into a waiting room and toilets.

[9] In 2008, there was a $1.5 million upgrade of the bus interchange,[10] with the subway to the station being rebuilt with a shallower grade, to allow wheelchair access.

A part of the project also involved the construction of a new broad gauge Platform 3, on the western side of the yard, which opened in November 2009.

It was moved to the current location in 1889, and a new nine-track, roundhouse-style shed was erected in timber and corrugated iron, along with a coal stage and brick offices.

Much of the roundhouse was removed in 1961 in conjunction with construction of the standard gauge line, and the rest of it was demolished in May 1971, leaving just the workshop buildings.

A goods shed was provided at Seymour in September 1873, of the same though-track, round-roof style seen at Kilmore East, Tallarook, Avenel, and Euroa stations.

[23] Until the 1960s, the main outbound traffic was agricultural produce, wool, firewood and timber, and there were incoming supplies for the town.

By the 1970s, small consignments of goods were only handled by a number of larger stations, with road transport used the rest of the way.

The Seymour Freight Centre opened on 8 February 1978, to serve the local area, and remained in use until 27 April 1985.

[27] Platform 1: Platform 2: Platform 3: Mitchell Transit operates five bus routes to and from Seymour station, under contract to Public Transport Victoria: Fallons Bus Service operates one bus route to and from Seymour station, under contract to Public Transport Victoria: V/Line operates road coach services from Seymour station to Albury, Echuca, Melbourne, Shepparton, Tocumwal and Wangaratta.