Sheephouse Wood is a 56.9-hectare (141-acre) biological Site of Special Scientific Interest east of Charndon in Buckinghamshire.

[1][2] The site has ancient pedunculate oak woodland with diverse ground flora, typical breeding birds and some uncommon invertebrates.

It is located on very poorly drained Oxford clay in the Vale of Aylesbury, and there are many small streams.

Invertebrates include the rare black hairstreak butterfly and ground-hopper tetrix subulata.

1.4 hectares of woodland at the southwestern edge of the site will be lost with the construction of High Speed 2, which will pass adjacent to the existing railway.