Yoshitora secretly trained Ana in the ways of the sohei (warrior monks of feudal Japan), so that she could seek out Arashi and avenge the deaths of her father and brother.
Arashi wanted to impress the leaders of the Osaka crime syndicate and left a calling card with every murder he committed—a simple coin engraved with the kanji for death; shi.
Whilst holding onto the coins left with the bodies of her father and brother, Ana developed a vengeful obsession, as she completely gave herself to shi, becoming the living embodiment of Death.
In order to disguise herself, Ana painted her face white to resemble Tora No Shi (also known as Tiger of Death), a legendary female warrior of medieval Japan.
However, Ana was deeply affected by her mother's Catholic teachings; she soon faced a dilemma between her programmed mission of revenge and the Christian faith she secretly harbored.
The first series depicted her conflict with Musashi Miyamoto, and the second centered upon her battle with Ieyasu Tokugawa during Yuri's time with the Japanese Christian resistance.