He was the younger son of Sir Benjamin Ayloffe and his second wife, Margaret the 5th daughter of Thomas Fanshawe.
[1] Ayloffe was a merchant in London, governor of the Russia Company from 1700, for the rest of his life; and was also active in local politics.
[2] In 1675, on the death of his elder brother Sir William Ayloffe, he inherited the baronetcy and family seat of Great Braxted.
[3] The baronetcy passed to his nephew (the son of his younger brother Henry), Sir John Ayloffe.
[1] They had one son, John, who died about the age of twenty, unmarried; and two daughters, Margaret, married to the Rev.