Sir John Shelton was the son of Sir Ralph Shelton (c. January 1431 – 16 July 1497) and Margaret Clere (d. 16 January 1500), daughter of Robert Clere, esquire, of Ormesby St Michael, Norfolk, and Elizabeth Uvedale, daughter of Thomas Uvedale, esquire.
By July 1536 Sir John was comptroller of the household established for Mary and Anne Boleyn's daughter, Elizabeth.
Sir John and Lady Shelton were given the joint title of Governor and Governess of the Princess Elizabeth, responsible for her upbringing and education, after her mother's execution.
[13][14] Shelton had three sons and seven daughters: Margaret, John, Mary, Ralph, Thomas, Anne, Gabriella, Elizabeth, Amy, and Emma.
[15] His son and heir, Sir John Shelton (b. in or before 1503, d. 1558), married Margaret, the daughter of Henry Parker, 10th Baron Morley.
His daughter Mary married first Sir Anthony Heaveningham, and second, Philip Appleyard,[17] the half-brother of Amy Robsart.