Sir John Vanden-Bempde-Johnstone, 2nd Baronet (28 August 1799 – 24 February 1869) was a British Member of Parliament.
In 1830 he was elected to the House of Commons for Yorkshire as a Whig, a seat he held until the constituency was abolished in 1832.
During the 1835 parliament he defected from the Whigs to the Conservatives, and lost his seat in 1837, but regained it in 1841, and continued to represent the constituency until his death 28 years later.
Vanden-Bempde-Johnstone married Louisa Augusta Venables-Vernon-Harcourt (1804–1869), daughter of the Most Reverend Edward Harcourt, Archbishop of York.
Lady Vanden-Bempde-Johnstone survived her husband by less than half a year, and died in August 1869.