Sofie Holten (1858–1930) was a Danish painter who created portraits, flower paintings and genre works.

In 1879, she went to Paris where she tried to assimilate the most modern methods of painting from teachers including Félix-Joseph Barrias and Alfred Stevens.

Holten exhibited her work at the Palace of Fine Arts at the 1893 World's Columbian Exposition in Chicago, Illinois.

In addition to her portrait of Strindberg, her Brudstykke af Parthenons Cellefrise (1901) and her painting of the Erechtheion Temple (1904) attracted attention.

In the late 1880s, she wrote reviews of female artists in Kvinden og Samfundet which was published by the Danish Women's Society.